Journal: Clinical and Developmental Immunology

Article Title: Both CD4 + and CD8 + Lymphocytes Participate in the IFN- γ Response to Filamentous Hemagglutinin from Bordetella pertussis in Infants, Children, and Adults

doi: 10.1155/2012/795958

Figure Lengend Snippet: Effect of the CD4 + or CD8 + depletion on the FHA-induced IFN- γ production by PBMC from B. pertussis -infected or vaccinated children. Isolated PBMC were stimulated in vitro for 72 h with 1 μ g/mL FHA after CD4 + (a) or CD8 + (b) depletion. Results from infected children are represented by columns 1 to 5 whereas those from vaccinated children are indicated by numbers 6 to 16. Open columns represent the values obtained for the nondepleted cell suspension and grey columns those obtained after CD4 + or CD8 + depletions. The arrows indicate the samples characterized by a higher IFN- γ secretion in CD8-depleted cell suspension compared to the PBMC.

Article Snippet: After fixation and permeabilization of the cells according to the manufacturers' instructions (Lysing-Solution1 and Permeabilizing-Solution2, BD Biosciences, USA), PBMC were stained with anti-human monoclonal antibodies: anti-CD3-PerCp-Cy5.5, anti-CD4-APC-Cy7, anti-CD8-APC, and anti-IFN- γ -PE (BD Biosciences, USA).

Techniques: Infection, Isolation, In Vitro